Microscopic Slice Image Processing and Automatic Labeling - Biomedical Signal Analysis: Contemporary Methods and Applications - page 353

Figure 13.2
Flow chart of the main counting steps during ZANE image analysis.
ROI detection when focusing through the slice) do not matter because
newborn cells sit in the subgranular layer, which is always included by
protocol. Hence a certain cell type is analyzed which is present only in
the given region. Other labeled cells in other regions are of another type,
which is not to be counted. These cells typically lie far enough from the
ROI region, which yields the low variability in ROI selection.