Environmental Engineering Reference
In-Depth Information
Chapter 2
A
M
ETA
-A
NALYSIS OF THE
B
ACTERIAL
AND
A
RCHAEAL
D
IVERSITY
O
BSERVED
IN
W
ETLAND
S
OILS
Junbao Yu
1,
, Xiaofei Lv
1,2
, Bin Ma
1
, Fanzhu Qu
1
, Kai Ning
1,2
and Huifeng Wu
1
1
Key Laboratory of Coastal Zone Environmental Processes and Ecological Remediation,
Yantai Institute of Coastal Zone Research (YIC),
Chinese Academy of Sciences (CAS),
Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes,
YICCAS, Yantai, P. R. China
2
University of Chinese Academy of Sciences, Beijing,
P. R. China
A
BSTRACT
This study examined the bacterial and archaeal diversity from a worldwide range of
wetland soils and sediments using a meta-analysis approach. All available 16S rRNA
gene sequences recovered from wetlands in public databases were retrieved. In
November 2012, a total of 12677 bacterial and 1747 archaeal sequences were collected in
Genbank. All the bacterial sequences were assigned into 6383 operational taxonomic
units (OTUs 0.03), representing 31 known bacterial phyla, predominant with
Proteobacteria (2791 OTUs), Bacteroidetes (868 OTUs), Acidobacteria (731 OTUs),
Firmicutes (540 OTUs), and Actinobacteria (418 OTUs). The genus Flavobacterium
(11.6% of bacterial sequences) was the dominate bacteria in wetlands, followed by Gp1,
Nitrosospira, and Nitrosomonas. Archaeal sequences were assigned to 521 OTUs from
phyla Euryarchaeota and Crenarchaeota. The dominating archaeal genera were
Fervidicoccus
and
Methanosaeta
. Rarefaction analysis indicated that approximately 40%
of bacterial and 83% of archaeal diversity in wetland soils and sediments has been
presented. Our results should be significant for well-understanding the microbial
diversity involved in worldwide wetlands.
