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Figure 3(B). Changes of ion content in mature seeding under salt stress.
Almost the same trends were discovered in mature seedlings (Figure 3.B).
The only difference was that the extent of the K + /Na + ratio lessened. In addition, the
content of the Na + and Cl - contents were abound in senile leaves.
3.4. The Behavior of Ion Flux under Salt Stress
In this experiment we adopted Non-invasive Micro-test Technique (NMT) (Figure 4A) to
test the ion flux. Firstly the typical flux patterns of Na + , K + , H + and Cl - were detected and the
specific situation was displayed in Figure 4B. The typical figures tell us that the Na + , K + , and
Cl - were excluded from the cells of roots, while the H + was shifted into the cells. The
experimental results (Figure 4C) of young seedling shows that with increasing salt
concentration, the Na + effluxes of BB52 were markedly increased and the maximum---52.4%
was reached at 300 mM NaCl.
Yet the ZH13 suffered the same stress did not display significant efflux ( P > 0.05). The
effluxes of K + were decreased with the increased NaCl, the content was dropped to 65.2% in
wild soybean exceeding 40.1% in cultivated one. Moreover there was visible difference in the
absorption of H + between them. The influx of H + was increased constantly in wild soybean
reaching 185.3% under 300 mM treatment, but the situation was not suitable for ZH13.
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