Biomedical Engineering Reference
In-Depth Information
Probe: 10 nM SSEA1
BSA
BSA
8
8
5
5
0
0
-3
-3
-3
0
50
120
-300
50
120
mES
miPS
8
8
5
5
0
0
-3
-3
-30
0
50
120
-30
0
50
120
mESD
miPSD
8
8
5
5
0
0
-3
-3
-30
0
50
120
-30
0
50
120
A19
293T
8
8
5
5
0
0
-3
-3
-30
0
50
120
-30
0
50
120
BSA
BSA
8
8
5
5
0
0
-3
-3
-30
0
50
120
Time (min)
-30
0
50
120
Figure 19.8
Real-time binding curves of the anti-SSEA1 antibody at 10 nm reacting with surface-
immobilized cells and bovine serum albumin (BSA) spots [38]. The vertical dashed lines are the
beginnings of association and dissociation, respectively. Reproduced from Lo
et al
., 2011 with
permission from BioTechniques.
surface markers may be at low densities on stem cells during differentiation, it is possible
that binding of the antibody is independent of the density and the variation in binding
affinity constants can be attributed to the variation in the presentation of the surface
markers [41].
