Nanopatterned Surfaces for Stem-Cell Engineering - Stem-Cell Nanoengineering - page 110

15.0kV 8.8 m ×100 k 500 nm 15.0kV 8.8 m ×100 k 500 nm

(C) (D)

15.0kV 8.8 m ×200 k 200 nm 15.0kV 8.8 m ×250 k 200 nm

Figure 7.21 Scanning electron microscopy images of (A) 20 nm and (B) 60 nm gold nanoparticle
immobilized indium tin oxide electrode. (C) and (D) are images of (A) and (B), respectively, at higher
magnification. Figure reproduced with permission from: ref. 73, © 2013 Elsevier.

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Figure 7.22 Proliferation of HB1.F3 cells seeded on different types of substrate. Approximately
2.1 × 10 4 cells were seeded on indium tin oxide (ITO), ITO/ arginine-glycine-aspartate (RGD) peptide,
ITO/20 nm gold nanoparticle (GNP), ITO/20 nm GNP/RGD peptide, ITO/60 nm GNP and ITO/60 nm
GNP/RGD peptide surfaces. Cells were counted by trypan blue proliferation assay after 72 h of
incubation in a common incubator. Figure reproduced with permission from: ref. 73, © 2013 Elsevier.