Biomedical Engineering Reference
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(C)
(D)
(E)
Figure 7.4 Neuronal in vitro differentiation of embryonic stem cells on an indium tin oxide
neurochip. (A and B) Overview showing enrichment of differentiating embryonic stem cells on
electrode surface: (A) day 2 post-seeding; (B) day 7 post-seeding. (C and D) At higher maginification:
(C) day 2 post-seeding; (D) day 7 post-seeding. (E) Immunostaining of MAP-2 (green fluorescence)
and synaptophysin (red fluorescence) at day 7 post-seeding. (For interpretation of the references to
color in this figure legend, the reader is referred to the web version of the article.) Figure reproduced
with permission from: ref. 39, © 2004 Elsevier.
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