Environmental Engineering Reference
In-Depth Information
taBlE 30.1
summary of common toxicity assays for different nanomaterials
Applications in
nanotoxicology
Assay
Purposes
Cell line
References
Neutral red uptake
Cell viability
Carbon nanotubes
A549 pulmonary cell line
OECd draft
guidance
129 [43]
Cell death
C
60
THP-I pulmonary cell line
ECvAM
validated [43]
Phototoxicity
Fe
3
O
4
Human monocyte macrophages
[44]
Trypan blue
Proliferative ability
Colloidal Ag
Bronchial epithelial cell
[45]
Cell viability
MoO
3
BEAS-2B
[46]
Cell growth
Fe
3
O
4
Rat liver cells BRL 3A
[47]
TiO
2
Human epidermal cells A431
[48]
Carbon-based nanoparticles
gold nanoparticles
Fluorescein diacetate
derivatives
Reactive oxygen
species
Copper oxide
Human epithelial cells HEp-2
[49]
TiO
2
Sd Primary hepatocytes
[50]
Lactate dehydrogenase
assay
disruptions in cell
membrane
Fe
3
O
4
Hepatocarcinoma cells Hep-g2
[51]
Metallic Cu
Kidney cells LLC-PK1
[52]
CuO
Rat liver cells BRL 3A
[46]
TiO
2
Metallic Ag
Titania stoichiometric
Metallic cobalt
Copper-zinc mixed oxide
variants
Nickel
Nickel oxide
Zirconia
Alumina
Tungsten carbide
CdO
MoO
3
MnO
2
Tetrazolium salts
(MTT, MTS)
Letal dose 50%
Fe
3
O
4
Hepatocarcinoma cells Hep-g2
[53]
Mitochondrial
activity
Metallic Cu
A549 pulmonary cell line
[51]
CuO
THP-I pulmonary cell line
[52]
TiO
2
Kidney cells LLC-PK1
Metallic Ag
Rat liver cells BRL 3A
Titania stoichiometric
Metallic cobalt
Copper-zinc mixed oxide
variants
Nickel
Nickel oxide
Zirconia
Alumina
Tungsten carbide
CdO
MoO
3
MnO
2
(
Continued
)
