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Table 7.1 Ligand-binding kinetics parameters for FixL and Dos
O 2
CN
CO
k on
( m M 1 s 1 )
k off
(s 1 )
K d
( m M)
k on
( m M 1 s 1 )
k off
(s 1 )
K d
( m M)
k on
( m M 1 s 1 )
k off
(s 1 )
K d
( m M)
References
10 4
10 4
Bj FixL
0.14
20
142
0.08
0.04
4.8
1.1
1.2
0.94 Gilles-Gonzalez et al. (2006)
Bj FixLH
0.3
10
33
-
-
-
-
-
-
Balland et al. (2005)
Bj FixLH
R220H
1.3
12
9
-
-
-
-
-
-
Balland et al. (2005)
Bj FixLH
R220A
1.4
1750 1250 -
-
-
-
-
-
Balland et al. (2005)
10 5
10 4
Sm FixL
-
-
33
-
-
-
2.6
3.1
12
Tanaka et al. (2006)
10 5
10 4
Sm FixLH
0.22
6.8
31
0.017
0.083 4.9
2.7
1.0
3.7 Gilles Gonzalez et al. (2006),
Winkler et al. (1996)
10 5
10 4
Sm FixLT
0.22
11
50
0.012
-
-
3.1
1.5
4.8 Gilles Gonzalez et al. (2006),
Winkler et al. (1996)
Ax PDEA1H 6.7
77
12
0.21
0.058 0.28
-
-
-
Chang et al. (2001)
Ec DosH
0.0026
0.034 13
0.0011
0.011 10
-
-
-
Chang et al. (2001)
Mt DosH
6
12
2
-
-
-
-
-
-
Tomita, Gonzalez, Chang, Ikeda-
Saito, and Gilles-Gonzalez (2002)
Sw Mb
14
12
0.86 0.51
0.019 0.037 -
-
-
Winkler et al. (1996)
Bj FixLH (residues 142-270); Sm FixL (residues 127-505); Sm FixLH (residues 127-260); Sm FixLT (residues 78-464).
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