Biology Reference
In-Depth Information
CHAPTER FOUR
The Globins of
Campylobacter
jejuni
Mariana Tinajero-Trejo
*
, Mark Shepherd
†
,1
*
Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, United Kingdom
†
School of Biosciences, University of Kent, Canterbury, United Kingdom
1
Corresponding author: e-mail address: m.shepherd@kent.ac.uk
Contents
1. Overview
99
2. NO and RNS in Biology
100
3. Oxygen and Reactive Oxygen Species in Biology
107
4. Respiratory Metabolism of Campylobacter
108
4.1 Microaerobic respiration
109
4.2 Respiration under oxygen-limited conditions
109
4.3 Impact of nitrosative stress upon Campylobacter
respiration
110
5.
Campylobacter Single-Domain Globin, Cgb: Functional and Structural
Characterisation
111
5.1 Functional characterisation
111
5.2 Cgb reduction: The redox partner conundrum
112
5.3 Structural characterisation
113
5.4 Biophysical and mechanistic characterisation
116
6.
Campylobacter
Truncated Globin, Ctb: Functional and Structural Characterisation
120
6.1 Functional characterisation
120
6.2 Structural characterisation
121
6.3 Biophysical and mechanistic characterisation
123
7. Control of Globin Expression
123
7.1 Control of globin expression by NssR
123
7.2 NssR classification, targets and mechanistic insights
126
7.3 Structural modelling of NssR
128
7.4 NrfA of Campylobacter
129
7.5 Induction of Cgb by nitrite and nitrate is independent of NrfA
130
7.6 NO resistance in oxygen-limited conditions
131
8.
Integrated Response Involving Cgb and ctb
131
9. Conclusions
132
Acknowledgements
133
References
133
