Biomedical Engineering Reference
In-Depth Information
Figure 10.10
Growth of PAMAM dendrimer
on the surface of MNPs for nonviral gene
transfection based on complexation with an
asODN. Nine steps are shown in the process:
3 - aminopropyltriethoxysilane (APTS) was
added to form amine-terminated MNPs
(G
0
dMNP), excessive methacrylate was
added to obtain ester-terminated MNPs,
ethylenediamine was added to form
amine - terminated G
1.0
dMNP, methacrylate
and ethylenediamine were added alternately to
obtain dMNP with generations from 1.0 to
5.0, complexation between dMNP and
asODN, adsorption of dMNP-asODN
complexes onto the cancer cell surfaces,
dMNP-asODN complexes were endocytosed
by cancer cells, endosome-containing
dMNP-asODN complexes were located
around the nucleus, and dMNP and asODN
escaped from the endosome into the
cytoplasm. Reprinted with permission from
Ref. [110]; © 2007, American Association for
Cancer Research.
