Biomedical Engineering Reference
In-Depth Information
Table 3.3
Reproducibility of magnetophoretic multiplexed immunoassays.
Immunoassay
Goat IgG - biotin
Rabbit IgG - biotin
Mouse IgG - biotin
Assay 1
Concentration (f
M
)
26.6
799.2
266.4
Multiplexed immunoassay (
μ
m)
17.00
±
3.00
96.45
±
5.09
59.44
±
5.48
Fitting into the standard curve (f
M
)
37.8
±
11.2
795.6
±
41.0
285.7
±
40.6
Assay 2
Concentration (f
M
)
666.0
399.6
6.7
Multiplexed immunoassay (
μ
m)
99.60
±
0.91
45.50
±
4.42
6.00
Fitting into the standard curve (f
M
)
682.6
±
11.8
381.2
±
37.2
4.5
Assay 3
Concentration (f
M
)
266.4
26.6
666
Multiplexed immunoassay (
μ
m)
56.80
±
5.97
9.10
±
3.00
98.70
±
3.46
Fitting into the standard curve (f
M
)
258.3
±
44.0
28.10
±
9.80
654.5
±
41.4
Accuracy of decoding - Assay 1: 100%, Assay 2: 93%, Assay 3: 93%.
No. of microspheres analyzed - Assay 1: 47, Assay 2: 30, Assay 3: 73.
Reproduced with permission from Ref. [88].; © 2008, Elsevier Ltd.
(a)
(b)
100
266.4 fM
133.2 fM
66.6 fM
26.6 fM
6.66 fM
100
3 uL/hr
5 uL/hr
7 uL/hr
80
80
60
60
40
40
20
20
n = 3
n = 3
0
0
0
200
400
600
800
1000
1200
1400
1600
1800
2000
A
B
C
D
Concentration of goat IgG-Biotin [fM]
Detection zone
Figure 3.16
(a) Effect of fl ow rate on the
dynamic range. Goat IgG-biotin was used,
and red microspheres that had been reacted
with goat IgG-biotin were detected at the
detection zone A; (b) Effect of the detection
zone on the dynamic range. Blue
microspheres that had been conjugated with
mouse IgG-biotin were measured at various
detection zones at a fi xed fl ow rate of 3
μ
l h
−
1
.
Four different detection zones were used; the
distance between the detection zones was ca.
4 mm. Reproduced with permission from Ref.
[88] ; © 2008, Elsevier Ltd.
