Real-Time Measurements of Phasic Changes in Extracellular Dopamine Concentration in Freely Moving Rats by Fast-Scan Cyclic Voltammetry - Drugs of Abuse: Neurological Reviews and Protocols - page 432

intracranial self-stimulation that provide information on dopamine's role in
reward. Next we show transient dopamine signals evoked by natural stimuli,
and fi nally, we present preliminary data demonstrating transient dopaminergic
neurotransmission during cocaine self-administration behavior.
2. Materials
2.1. Electrochemistry
1. Multifunction input/output card: PCI-6052E (16 bit, 333 kHz) (National Instru-
ments, Austin, TX).
2. Virtual instrumentation software: Locally written in LabVIEW (National Instru-
ments).
3. Potentiostat: EI-400 (Cypress Systems, Lawrence, KS).
4. Headstage: Miniaturized current-to-voltage converter (UNC-CH Electronics
Design Facility, Chapel Hill, NC).
5. Swivel: CAY-675-12 (12 electrical and 1 fl uid channel) (Airfl yte, Bayonne, NJ).
The software we use for fast-scan cyclic voltammetry and data analy-
sis will be marketed with data acquisition hardware and a potentiostat for
fast-scan cyclic voltammetry: FAST-FSCV16, Quanteon, Lexington, KY
(http://quanteon.cc/).
2.2. Electrical Stimulation
1. Digital-to-analog card: PCI-6711 (National Instruments).
2. Constant-current stimulator: Neurolog NL800 (Harvard Apparatus, MA).
2.3. Electrode Fabrication
1. Carbon fi ber: T-650 (Thornel, Greenville, SC).
2. Capillary glass: 6245 (0.6 mm outer, 0.4 mm inner diameter) (A-M Systems,
Carlsborg, WA).
3. Vertical electrode puller: PF-2 (Narishige, Tokyo, Japan).
4. Micromanipulator ( Fig. 2 ) (UNC-CH Machine shop).
5. Silver paint: 22-0202-0000 (Silver Print) (GC Electronics, Rockford, IL).
6. Heat shrink tubing: KYNAR 3/64-inch diameter, (3 M Electronics, Austin, TX).
7. Activated carbon: 102489 (NORIT A decolorizing carbon) (ICN Biomedicals,
Aurora, OH).
8. Silver wire: 32,702-6 (0.5 mm diameter) (Sigma, St. Louis, MO).
9. Gold connector: 46N6790 (socket pin terminal) (Newark Electronics,
Chicago, IL).
2.4. Surgery
1. Anesthetic: K-113 (ketamine HCl/xylazine HCl solution) (Sigma).
2. Stereotaxic frame: Model 900 Small Animal Stereotaxic (David Kopf Instru-
ments, Tujunga, CA).