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Fig. 1. Induction of Fos protein by dopamine D1 receptor-selective agonist
SKF-81297 in striatal slice culture.
(A)
Immunostaining of Fos protein showing
induction of Fos by SKF-81297 (100 n
M
) in striosomes of striatal slice culture
derived from newborn rat brain. The slice was cultured for 3 d before treatment
with SKF-81297. The bracketed region in
A
is shown at higher magnifi cation in
C
.
Clusters of Fos-positive nuclei (examples at
asterisks
) that represent striosomal loci
are distributed through the striatum. Fos-positive nuclei are also present in the medial
compartment (MC).
(B)
Photomicrograph of Fos immunostained striatal slice that
was cultured for 3 d in parallel with the slice shown in
A
, but was pretreated with the
D1-selective dopamine receptor antagonist, SCH-23390 (1
M
), before SKF-81297
treatment. The SCH-23390 pretreatment blocks the striatal Fos induction by SKF-81297.
The bracketed region in
B
is shown at higher magnification in
D
. AC, Anterior
commissure. Scale bar in
B
(for
A
and
B
) indicates 500
µ
µ
m. Scale bar in D (for
C
and
D
) indicates 100
m. (Reproduced from Liu et al.
[7]
with permission. © 1995
The Society for Neuroscience.)
µ
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