Biomedical Engineering Reference
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Fig. 3. Fold changes in Cx32 expression in the medulla, parietal/temporal cortex,
cerebellum, and thalamus.
(A)
Medulla.
(B)
Parietal/temporal cortex.
(C)
Cerebellum.
(D)
Thalamus. Note that there is no change in Cx32 expression in the medulla or
parietal/temporal cortex relative to drug-naive animals during cocaine withdrawal.
A transient increase in expression is observed 2 d after cocaine withdrawal in the
cerebellum while sustained increases in Cx32 expression are noted in thalamic nuclei
2-21 d after cocaine withdrawal.
ELISA protocol depends on use of high-affi nity antibodies with minimal cross-
reactivity to other proteins and reagents and it is worth the time to optimize
these reagents.
3. If the absolute amounts of protein are to be quantifi ed, then a standard curve with
serial dilutions of known amounts of target protein is required. However, binding
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