Quantification of Protein in Brain Tissue by Western Immunoblot Analysis - Drugs of Abuse: Neurological Reviews and Protocols - page 265

and secondary antibodies; (d) dilute samples and load less protein per lane; and
(e) try a less sensitive chemiluminescent detection system
11. Stripping and reprobing blots: If membrane is dry, soak in methanol for 2 min.
Rinse blots with PBST. Incubate for 20 min at 50°C in a solution containing: 2%
SDS; 100 m M -mercaptoethanol; and 50 m M Tris-HCl, pH 6.8. Wash 3 × 5 min

in PBST before reprobe by starting with the blocking step.

References
1. Harlow, E. and Lane, D. (1998) Using Antibodies: A Laboratory Manual . Cold
Spring Harbor Laboratory Press, Cold Spring Harbor, NY.