Biomedical Engineering Reference
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Finally, never perform an experiment with RNA that has not been carefully and
fully characterized.
2. Shaking is such an important step in the process that it bears mentioning again.
During both prehybridization and hybridization, shaking ensures that “clouds” do
not appear at corners of edges of the membranes, which can complicate analysis.
Examples of this problem are illustrated in
Fig. 3
.
Acknowledgments
This work was supported by NIH Grants R01-DA13770, P50-DA06634,
P50-AA15773 (to K. E. V.), T32-DA07246 (to W. M. F.), and T32-AA07565
(to T. J. W.).
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