Biomedical Engineering Reference
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Fig. 1. Coupling
in situ
hybridization with retrograde tracing. Double labeling with
FluoroGold and D1 or D2 dopamine receptor cRNA probes in the prefrontal cortex
following FluoroGold (4% in saline) injection in the striatum.
(A
,
C)
FluoroGold-
labeled neurons in the layer V of the prefrontal cortex. (
B
,
C)
show double-labeled
neurons visualized by combining dark-fi eld (silver grains) with epifl uorescence as in
A
and
C
for FluoroGold.
White arrows
point to neurons containing both FluoroGold
and D1 or D2 mRNAs in
B
and
D
, respectively. The
open arrows
point to neurons
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