Environmental Engineering Reference
In-Depth Information
(a)
(b)
Per1
gene
transcriptional response
GFP-GR
9
8
7
6
5
4
3
2
1
0
DAPI
Overlay
DMSO
SS97
Corticosterone
(c)
Abundance [1335] 10 targets (t)
100
CH
3
2
CH
3
CH
3
H
CH
3
CH
3
H
H
H
75
CH
3
O
H
H
HO
O
CH
3
CH
3
H
H
3
CH
3
H
H
H
50
O
OH
H
H
H
1
H
3
C
CH
3
25
0
8.30
8.53
8.77 9.00
9.24 9.47 9.71
9.94
10.26
10.57
10.88
11.20
11.51
11.82
12.14
12.45
12.76
Time
(d)
Overlay
GFP-AR
DAPI
FIGURE 35.6
Analysis of water sample SS97 for glucocorticoid and androgen contamination. (a) Sample SS97 collected by a
POCIS induces complete GFP-GR translocation in 30 min. Scale bar, 5 μm. (b) Transcriptional activation of the
GR-regulated
Per1
gene by sample SS97 (concentrated 100×) is compared with transactivation induced by corti-
costerone. Data are normalized to the solvent (DMSO). Error bars represent the mean ± s.e.m., n = 3. (c) GC/MS
total ion chromatogram of one of the HPLC fractions of sample SS97 revealed the presence of a complex mixture
of volatile hydrocarbons, as indicated by the peaks. Database searching of the extracted MS spectra correspond-
ing to peaks 1-3 showed structural similarity to known androstane-type steroids. (d) Representative images of
GFP-AR nuclear translocation in response to 100 nM of testosterone, androst-4-ene-3,6-dione, and sample SS97
(100× concentrated). Scale bar, 5 μm.
