Evaluation of a Zinc Clinoptilolite for Drinking Water Treatment - Aquananotechnology: Global Prospects - page 608

TABLE 30.1
Bacterial Strains and Yeasts Removed by ZZ

Bacterial Strains and Yeasts
Bacillus subtilis ATCC 6633

Streptococcus cloacae ATCC 23355 Bacillus cereus BSG 001

Pseudomonas aeruginosa 2327 Salmonella typhimurium ATCC 14628 Streptococcus freundii BSG 013

Pseudomonas aeruginosa ATCC 27853 Salmonella anatum BSG 012 Serratia marcescens ATCC 8100

Klebsiella pneumoniae BSG 028 Salmonella typhimurium BSG 030 Arizona ATCC 67344

Klebsiella pneumoniae BSG 036 Proteus mirabilis BSG 013 Citrobacter freundii BSG 032

Vibrio cholerae No. 01 Proteus vulgaris BSG 015 Providencia ISA 21

Staphylococcus aureus ATCC 25923 Proteus vulgaris ATCC 13315 Klebsiella pneumoniae BSG 003

Staphylococcus epidermidis BSG 021 Proteus rettgeri ATCC 1407 Klebsiella pneumoniae ISA

Shigella sonnei ATCC 75931 Escherichia coli 44 Candida albicans BSG 002

Shigella lexneri ATCC 12027 Escherichia coli ATCC 25922 Candida albicans BSG 003

Shigella lexneri BSG 009 Escherichia coli ATCC 25932 Candida albicans BSG 007

50 mL of LB media and 10% ZZ. Additionally, a control Erlenmeyer was inoculated
that contained neither of these products. After introducing ZZ, the test proceeded in
two ways: stirring and not stirring or static. The bacterial strains used in the test were
selected considering their virulence and survival span in drinking water, as presented
in Table 30.2.
The test conducted to evaluate the effect of ZZ on Giardia lamblia cysts was performed
according to the following procedure:

• Extraction of G. lamblia cysts from infected human feces
• Cyst count using Neubauer chamber
• Production of the infective dose (20,000 cysts/mL)
• Inoculation of the Erlenmeyers containing drinking water control sample and
those with ZZ 10%
• Cyst count after 30 min, 1 h, and 3 h

TABLE 30.2
Bacterial Strains Used to Test the Bactericidal Effect of ZZ in Drinking Water

Bacteria Strain Characteristics

Vibrio cholerae C7258 Serotype Ogawa, epidemic

C6706 Serotype Inaba, epidemic

Pseudomonas aeruginosa O11 Serotype O11, reference

5FQ Serotype O9, isolated from cystic ibrosis patients

Leptospira interrogans M20 Serovar Copenhageni serogroup icterohaemorrhagiae, reference

Shigella sonnei
Salmonella typhi
Aeromonas hydrophila ATCC 7614

Plesiomonas shigelloides CNCTC 5132

Escherichia coli O 149 K88 Lt(+)

Escherichia coli O 101 K99 St(+)

Escherichia coli ATTC 25922