Biology Reference
In-Depth Information
Application of this method to the HPLC analysis of plant extracts
discriminates the dehydroellagitannins from other tannins (Fig. 4.16).
The HPLC peaks attributed to dehydroellagitannins disappear after
treatment of the extracts with aqueous acetone containing ammonium
formate, and instead, sharp peaks arising from the acetone adducts
appear at longer retention times.
OH
OH
HO
HO
OH
OH
OH
HO
O
SH
H
O
OH
O
O
O
MeO
2
C
NH
2
geraniin
O
O
r.t.
O
O
O
H
S
O
MeO
2
C
H
acetone
adduct
HN
H
OH
O
O
OH
HO
SH
H
heat
MeO
2
C
NH
2
r.t.
OH
OH
OH
HO
OH
HO
HO
OH
HO
OH
OH
OH
OH
OH
HO
O
HO
O
O
OH
O
O
O
O
OH
O
O
O
O
O
O
O
corilagin
OH
OH
O
O
O
H
S
O
+
O
MeO
2
C
H
S
O
OH
N
OH
MeO
2
C
HN
OH
HO
OH
OH
HO
O
O
Fig. 4.17 Reaction of geraniin and its acetone adduct with
L
-cysteine methyl ester.
