Chromatographic Methods for Environmental Analysis - Fundamentals of Environmental Sampling and Analysis - page 285

Martin (AJP) and Synge (RLM) (1941), A new form of chromotogram employing two liquid phases. I. A
theory of chromotography. 2. Application the micro-determination of the higher Monoamino-acids in
proteins. Biochem. J. 35: 1358-1368.
*M C N AIR HM, M ILLER JM (1998), Basic Chromatography, John Wiley & Sons, Inc., New York, NY.
M EYER VR (1997), Pitfalls and Errors of HPLC in Pictures,H ¨ thig Verlag Heidelberg.
M EYER VR (2004), Practical High-Performance Liquid Chromatography, 4th Edition, John Wiley & Sons,
NY.
O'D ELL JW et al. (1984), The Determination of Inorganic Anions in Water by Ion Chromatography-
Method 300.0. EPA-600/4-84-017, Environmental Monitoring and Support Lab, Cincinnati,
OH.
R UBINSON KA, R UBINSON JF (2000), Contemporary Instrumental Analysis, Prentice Hall, Upper Saddle
River, NJ.
*S ADEK PC (2000), Troubleshooting HPLC Systems: A Bench Manual. John Wiley & Sons, Inc., NY.
S KOOG DA, H OLLER FJ, N IEMAN TA (1997), Principles of Instrumental Analysis, 5th Edition, Saunders
College Publishing, NY.
S NYDER LR, K IRKLAND JJ, G LAJCH JL (1997), Practical HPLC Method Development, 2nd Edition, John
Wiley & Sons, Inc., NY.
QUESTIONS AND PROBLEMS
1. Define the following forms of chromatography: GSC, GLC, LSC, and LLC. Which one
of these four chromatographic techniques is commonly referred to as GC? Which one is
commonly referred to as LC?
2. Describe (a) the difference between adsorption-based chromatography and partition-
based chromatography, and (b) the common stationary phases for GLC and LLC.
3. What differs between three capillary columns, WCOT, SCOT, and PLOT?
4. What differs between packed columns and capillary columns regarding their perfor-
mance in separation? Why?
5. Describe the following (a) Why temperature is important for a good separation in GC,
but not in mobile phase? (b) Why solvent mobile phase is important for a good
separation in HPLC but not temperature?
6. Describe the effect of the following variables on the resolution and analytical speed
(length of retention time) in GC: (a) column diameter, (b) film thickness, and (c) column
length.
7. List three methods to improve the resolution in HPLC.
8. In selecting GC column temperature, what factors need to be considered?
9. To separate very polar components by HPLC, would you choose a very polar or non-
polar material for the stationary phase? Which component (most polar to least polar) will
be eluted first?
10. Define the following terms: separation factor, retention factor, resolution, plate number,
and plate height. Which one best defines the column efficiency?
11. Explain why resolution and speed of analysis is always a compromise during a
chromatographic analysis.
12. Why a capillary column is more efficient than a packed column in separation?
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