Chemistry Reference
In-Depth Information
our level of understanding is increasing rapidly, none of the molecular motors have
been solved.
Further advances in
fluorescent probes, instrumentation and biological assays
will accelerate progress. Techniques for introducing some of the complexity of the
cell into in vitro studies and for collecting high resolution signals within live cells are
beginning to emerge. Single-molecule imaging is likely to impact over a broad
segment of cell biology as novel methods for collecting hyper-resolution images
become more practical and images that give truly molecular insight become
available. Watching and understanding every important molecule in a cell do its
job is farfetched, but the tools are being assembled to enable us to consider what
would be required and how close we can get to that goal.
Acknowledgements
I thank the members of my laboratory for the productive and enjoyable environment,
Drs. P.R. Selvin andA. Yildiz for permission to use unpublished data, Yujie Sun, John
Lewis and John Beausang for helpful comments on the manuscript, and the NIAMS,
NIGMS and NSF for research support.
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