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shown in C). (C) 50mMMg
2 รพ
was used to slow
down the reactions here but measurements at
lower concentrations showed that only the
absolute rates increased without any change in
relative populations of branch points and
conformations. (D) FRET histograms within slow
and rapid phases show that two branch points
have distinct FRET values for the high FRET
conformations. Adapted from [15].
Figure 11.7 Mon-migratable HJ. (A) Each of the
two brabch points (U and M) will fold into two
conformations. For this particular sequence one
branch point (U) is 20 times more populated
than the other (M) due to sequence-dependent
stability. (B) Single-molecule FRET trace shows
two phases, a slowly fluctuating phase with a bias
to the high FRET state (U) and a rapidly
fluctuating phase without a bias (M, detail is
