Chemistry Reference
In-Depth Information
Figure 8.3 Dynamics of single mRNA molecules
in the cytoplasm of mammalian cells. Direct
movements are also observed in the cytoplasm.
(A) Cos cells transiently expressing the reporter
hGH mRNAs and the MCP-GFP were imaged
live. Left: a maximum intensity image projection
of 200 frames on one image. The scale bar
represents 10
boxed regions. The blue arrow points to a
static particle in the vicinity of a corralled mRNA.
(B) COS cells transiently expressing the reporter
SV mRNA and MCP-GFP were imaged as in A.
The scale bar represents 10
m. Right: panel
magnifications, track of mRNA movement
superimposed (green) on an enlargement from
each of the indicated boxed regions. The scale
bar represents 2
m
m. Right: panel magnifications:
the scale bar represents 2
m
m. mRNA track
superimposed (green) fromeach of the indicated
m
m
m. (Adapted from [57]).
or with adaptors, a speci c outcome will be determined as the RNA distribution will
be a result of the motor direction and its persistence.
8.4.2
RNA Localization
RNA localization is involved in the regulation of many processes inside the cell and
often acts in concert with translation and RNA degradation. Most of its effects give
 
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