Chemistry Reference
In-Depth Information
Non-integral membrane receptor
Con A
in Saccharomyces
Glucose
Mannose
Gold-coated AFM probes were
functionalized with oligoglucose
or Con A using thiol chemistry.
Individual lectin
carbohydrate interactions were recorded in
living yeast cells in aggregating conditions. Unbinding forces
of about 120 pN have been measured at a rate of 0.5
-
[184]
strains
ms
1
.
m
Cells were immobilized by me-
chanical trapping in a polycar-
bonate membrane
csA
in aggregating cells of
Dictyostelium discoideum
csA
in aggregat-
ing cells of Dic-
tyostelium
discoideum
Tipless cantilevers were functio-
nalized with a lectin for
The EDTA-stable cell adhesion was mediated by the csA
glycoprotein. A rupture force of 23 pN at a separation rate of
2.5
[185]
rm
ms
1
was measured.
attachment of a cell
The targeted cells were grown in
Petri dishes
m
Abbreviations for proteins: Con A, concanavalin A; csA, contact site A; LFA-1, leukocyte function-associated antigen-1; PSGL-1, P-selectin glycoprotein ligand-1; SGLT,
sodium/glucose co-transporter.
Other abbreviations: BSA, bovin serum albumin; CHO, Chinese hamster ovary; EGTA, ethylene glycol-bis(2-aminoethylether)-N,N,N
0
,N
0
-tetraacetic acid; HUVECs,
human umbilical vein endothelial cells; ICAM-1, intercellular adhesion molecule-1; ICAM-2, intercellular adhesion molecule-2; IgG, immunoglobulin G; PEG, poly
(ethylene glycol); PMA, phorbol myristate acetate; PMN, polymorphonuclear leukocyte; VCAM-1, vascular cell adhesion molecule-1.
