Chemistry Reference
In-Depth Information
a
V
b
3
integrin
receptor
in osteoclast and
osteoblast cells
RGD
peptides and
proteins
The cantilever tips were coated
with PEG prior to passive
chemisorption of ligands
Rupture forces ranged between 40 and 100 pN for the different
ligands. Separation rates were reduced from 50 to 1
[176]
ms
1
.
m
Cells were
xed on glass cover
slides
b1 integrin in
endothelial cells
(HUVECs) and
leukemia HL-60
cells
P-selectin
E-selectin
ICAM-1
VCAM-1
Individual HL-60 cells were at-
tached to the tip of a cantilever
through Con A-mediated linkage
Soluble forms of VCAM-1 and P-
selectin were adsorbed onto Petri
dishes
Endothelial cells were grown on
The detachment of leukocyte
-
HUVEC complexes involved a
series of rupture events with force transitions between 40 and
100 pN at a retraction speed of 3
[177]
ms
1
. Similar rupture
magnitudes were observed on immobilized P-selectin and
VCAM-1 proteins. Adhesion was inhibited by cyclic RGD
peptides.
m
bronectin-coated Petri dishes.
ms
1
was
Fibronectin adhesin
in
Staphylococcus epidermis
bacteria
Fibronectin
Fibronectin molecules were
bound to gold-coated AFM tips
by chemisorption of thiol groups.
A rupture force of 85 pN at a separation rate of 1
m
[178]
measured. Speci
c interactions are located at the heparin-
binding site of
bronectin.
Bacteria were grown on
bro-
nectin-coated surfaces.
A single linear regime in the force spectrumwas observed. Bell
model parameters were k
d
(0)
4.8 s
1
and x
b
¼
0.33 nm. The
value of k
d
(0) was six orders of magnitude larger than that
estimated for the thermally activated dissociation, which
demonstrated that the potential energy curve of the interaction
was controlled by more than one activation barrier
Low adhesion probability at short contact time, and
k
a
c
eff
¼
¼
11.8 s
1
.
(Continued)
