Chemistry Reference
In-Depth Information
b1 integrin
m
beads coated with antibody in
sympathetic neurons
tagged with 0.2-
m
GRGDS peptide
SPT
Diffusion: The effect of NGF on the movement of
1
integrin, a subunit of the receptor for laminin and
bro-
nectin, was studied. Binding of GRGDS peptide to
b
[162]
1
integrin caused rearward movement. NGF stimulated
rapid displacement of unliganded (low-crosslinked) re-
ceptors and forward excursions toward the tips of
b
lopodia.
Forward transport was dependent on actin
laments and
myosin ATPase activity. NGF stimulated the coupling of
crosslinked
b
1 integrins to the retrograde
ow of actin.
b1 integrin
tagged with 40-nm
gold particles coated with an-
tibody or
RGD peptide
Fibronectin
SPT
Diffusion: The effect of RGD peptide or
bronectin binding
to integrins on the receptor lateral diffusion was studied.
Ligand binding regulates integrin association with dy-
namic components of the cytoskeleton, which is crucial for
directed receptor movement. Directed motion was medi-
ated by crosslinking between the cytoplasmic domain of the
b
[163]
bronectin in
broblasts
1 subunit and the rearward-moving cytoskeleton.
Class II MHC receptor
in CHO
Moth Cytochrome C peptide
labeled with Cy5
SMI
Diffusion: Both GPI-linked and native class II MHC re-
ceptors exhibit predominant Brownian motion with no
in
[164]
cells
uence of actin or tubulin cytoskeletal networks.
EGFR
in human diploid
EGF
labeled with rhodamine
FCS
Diffusion: Two populations of ligand
receptor complexes
with different mobility, representative of different forms
(dimers, oligomers) or subtypes of EGFR.
[165]
-
broblasts
Binding: Equilibrium saturation binding experiment
yielded K
d
¼
0.7 nM. The dissociation kinetics follow a
single exponential function with k
diss
¼2.9 · 10
4
s
1.
(Continued)
