Chemistry Reference
In-Depth Information
Table 7.1
(Continued)
Receptor
Ligand
Technique
Receptor mobility and ligand binding properties
Reference
OR17-40
fused to the acyl car-
rier protein and
uorescently
labeled with CoA-Cy5 in HEK
293 cells
Agonist
helional
Antagonist a-methyl-
cinnamaldehyde
SMI
Diffusion: Regulation of the internalization dynamics of
OR17-40. Both agonist and antagonist induced con
ne-
ment in
[149]
190-nm-sized domains which are probably
precursors of clathrin-coated pits.
SSTR1
and
SSTR5
in CHO-K1
Somatostatin
labeled with
FITC or Texas red
FRETand FCS
Diffusion: SSTR5 homooligomerization and SSTR5/SSTR1
heterooligomerization was regulated by ligand binding.
[150]
cells
Ion channels
5-HT
3
R
in HEK 293 cells
Antagonist
GR
labeled with
FCS
Diffusion: The majority of the clustering receptors are
immobile, a minority shows diffusion.
[151]
Cy5
5-HT
3
R
tagged with a polyhis-
tidine sequence and labeled
with Ni
2þ
-NTA-Atto 647 in
HEK 293 cells
Antagonist
GR
or GR labeled
SMI
Diffusion: Serotonin slightly decreased the fraction of
mobile receptors.
[152]
with Cy5
Agonist
serotonin
AMPAR
containing the gluta-
mate receptor subunit GluR2
in cultured hippocampal neu-
rons and tagged with 0.5-
Glutamate
SPT
Diffusion: Regulation of diffusion by neuronal maturation,
synaptic sites, basal neuronal activity (action potentials,
intracellular calcium) and activation with glutamate. The
mobility of GluR2 was slowed down by glutamate and
calcium.
[153]
m
latex beads coated with anti-
GluR2 antibodies
m
