Chemistry Reference
In-Depth Information
C-peptide receptor
in human
C-peptide
labeled with
tetramethylrhodamine
FCS
Diffusion: Two populations of ligand
receptor complexes
[142]
-
skin
broblasts
with different mobility.
Binding: C-peptide binding isotherm yielded K
d
¼
0.3 nM.
Functional C-peptide receptors could be solubilized with
Chaps detergent.
C-peptide receptor in tubular
cells
C-peptide labeled with
tetramethylrhodamine
FCS
Binding: Displacement of bound tetramethylrhodamine-
labeled C-peptide by synthetic C-peptide fragments. Resi-
due Glu27 at the C-terminal part is particularly important
for speci
[143]
c binding.
ET
A
R
in virus-like particles
Endothelin-1
labeled with
tetramethylrhodamine
FCS and FIDA
Binding: Equilibrium binding experiments yielded
K
d
[144]
0.3 nM similar to values measured in living cells.
Galanin receptor
in cultured
Galanin
labeled with
tetramethylrhodamine
FCS
Diffusion: Two populations of ligand
receptor complexes
with different mobility that might belong to different
subpopulations or receptor subtypes.
[145, 146]
-
insulinoma cells
Binding: Binding isotherm yielded one binding site with
K
d
¼
1). Displacement experiments yielded a
single dissociation rate constant k
diss
¼
1.2 nM (n
¼
10
4
s
1
.
Pertussis toxin abolished binding indicating that galanin
binds to a GPCR.
3.7
·
mGluR5
in hippocampal neu-
rons and Ptk2 cells tagged with
0.5-
DHPG
SPT
Diffusion: Regulation of mGluR5 diffusion by the sub-
membranous scaffolding protein Homer and by receptor
activity. In presence of DHPG the diffusion coef
[147]
m latex beads coated with
anti-myc antibodies
m
cient was
increased by a factor of three but the relative distribution of
diffusive and con
ned states was not modi
ed.
NK1R
labeled with EGFP in
HEK 293 cells
Tetramethylrhodamine-la-
beled
SMI
Binding: The mobility of the ligand
receptor complexes
[148]
-
substance P
was slowed down during the
rst 1 s after ligand binding
and became more con
ned.
(Continued)
