Chemistry Reference
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Figure 6.4 Analysis of single particle tracking
data taken with an emCCD camera. (a)
Sequential frames from a time series. Yellow
spots indicate successful tracking. Blue spots
indicate quantum dot off states. Only spots
with a sufficiently high correlation to the
microscope PSF were chosen for tracking.
Quantum dots with 655-nm emission were
imaged using an Andor iXon emCCD camera
with illumination from a Hg lamp (435/20
bandpass filter) using 20-ms exposures at
33 frames/s.) (b) The intensity trace of the
indicated QD over time demonstrates blinking.
(c) Trajectory (blue) of the indicated (arrow) QD
in A. Filled (red) circles designate off periods.
(d) Diffusion constants were retrieved by fitting a
parameterized model to the Mean Square
Displacement (MSD) curve. (e) Distribution of
diffusion constants found for receptors on the
cell body (red) or filopodia (blue). Diffusion
constants are derived by a linear fit to the first 10
points (0.3 s) of the MSD curve.
spectroscopy. Other reported PAMs are based on DMD [14 - 17] and liquid crystal-
on-silicon (LCoS) [18] SLMs. Of these, only one [15] was applied in uorescence
microscopy, using a fringe projection technique similar to that used by Wilson
et al. [19]. The DMD-based PAM suffered from several serious limitations leading to
 
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