Genome Engineering by Transgene- Instructed Gene Conversion in C. elegans - Caenorhabditis Elegans: Molecular Genetics and Development - page 86

phenotype caused by the MosTIC allele. This protocol was successfully used to
engineer point mutations, KO/del and KI alleles.
The use of MosTIC-engineered alleles is increasing and it has been demonstrated
that (i) they are compatible with genetics, cell biology, and biochemistry approaches,
(ii) their use minimizes experimental artifacts due to misexpression of transgenic
sequences in C. elegans, and (iii) gene tagging by homologous recombination can
bypass the time consuming and expensive task of developing specific antibodies
against each protein of interest.
Acknowledgment
The authors thank Luis Briseno-Roa and Berangere Pinan-Lucarre for critical reading of this
manuscript.
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