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Fig. 2 Description of the standard MosTIC procedure. (A) Overview of the standard MosTIC protocol (see text for details).
(B) MosTIC efficiency using the standard procedure. Examples of MosTIC-engineered alleles including point mutation, KO/del
or KI alleles. They were recovered at frequency ranging from 10 -3 to 10 -5 events per offspring. Note that reducing the length of
one homologous arm decreased MosTIC efficiency (fourth example). (C) MosTIC conversion tract (adapted from Robert and
Bessereau, 2007 ). The region that can be efficiently converted by MosTIC spans 1 kb centered at the Mos1 insertion point. See
text for details. (See color plate.)
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