Molecular and Genetic Approaches for the Analysis of C. elegans Neuronal Development - Caenorhabditis Elegans: Molecular Genetics and Development - page 421

Table III
Antibody toolkit for C. elegans

Markers for Antibodies against Reference

Synaptic vesicles Synaptobrevin (SNB-1) (Hadwiger et al., 2010)

Synaptic active zones Rim (UNC-10) (Hadwiger et al., 2010)

Synaptic active zones SYD-2 ( Zhen and Jin, 1999 )

Synaptic active zones UNC-13 ( Charlie et al., 2006 )

Synaptic periactive zones RPM-1 ( Abrams et al., 2008 )

Postsynaptic regions (GABAergic neurons) GABA receptor (UNC-49) ( Gally and Bessereau, 2003 )

Postsynaptic regions (cholinergic neurons) Nicotinic acetylcholine receptor (UNC-29) ( Gally et al., 2004 )

Postsynaptic regions (cholinergic neurons) Nicotinic acetylcholine receptor (LEV-10) ( Gally et al., 2004 )

Recycling endosome EHD1 (RME-1) (Hadwiger et al., 2010)

Endoplasmic reticulum The cytochrome P450 (CYP-33E1) (Hadwiger et al., 2010)

Golgi Beta-1,3-glucuronyltransferase (SQV-8) (Hadwiger et al., 2010)

Mitochondria Chaperonin (HSP-60) (Hadwiger et al., 2010)

Lysosomes LAMP (LMP-1) (Hadwiger et al., 2010)

Clathrin-mediated endocytosis sites markers Dynamin (DYN-1), the alpha-subunit of the

adaptor complex 2 (APA-2)

(Hadwiger et al., 2010)

Inhibitor transmitter (GABA) GABA ( McIntire et al., 1993 )

Excitatory transmitter (5-HT) Serotonin (5-HT) ( Weinshenker et al., 1995 )

Touch neurons Acetylated alpha-tubulin ( Siddiqui et al., 1989 )

GABAergic neurons Beta-tubulin (one isoform) ( Siddiqui et al., 1989 )

Cholinergic neurons Choline acetyltransferase (ChAT)/ UNC-17 ( Duerr et al., 2008 )

Dopaminergic neurons Dopamine transporter (DAT-1) ( McDonald et al., 2007 )

C. Electron Microscopy (EM)
Serial-section EM has been used to reconstruct the cellular architecture of the
C. elegans nervous system. The brilliant work of JohnWhite using serial-section EM
had provided us with the structure and connections of the worm nervous systems.
Detailed methods about EM can be found in the chapter of this topic by David Hall.

IV. Functional Dissection of Signaling Pathways Controlling
Neuronal Development

Rationale: To study the cellular processes and dissect the molecular signaling
pathways underlying neuronal development, the first step is to characterize the
normal developmental features and the second step is to identify and characterize
mutants that affect the phenotypes. Further molecular and genetic manipulations
will result in a comprehensive understanding of the signaling mode and pathways of
the gene of interests. Below, we outline the general uses of transgenic labeling
approaches to analyze of neuronal development.