Molecular and Genetic Approaches for the Analysis of C. elegans Neuronal Development - Caenorhabditis Elegans: Molecular Genetics and Development - page 417

presynaptic terminals using SNB-1:: GFP ( Nonet et al., 1998 ) has led to the
generation of multiple labeling of different synaptic domains using fluorescent
fusion proteins. Table I I lists many well-characterized transgenic lines that label
presynaptic terminals and postsynaptic sites of different neurons.

C. Complex Strategy for Labeling and Manipulating Unique Neurons
Finding promoters that are activated in multiple neurons is usually straight for-
ward, while it remains challenging to obtain promoters that are uniquely specific for

Table II
Transgenic lines for labeling neurons synapses and compartments

Transgenic
worms

Plasmids Labeled neurons Linkage Reference

hpIs3 Punc-25-GFP::SYD-2 DD VD neurons presynaptic active

zones

LGX ( Ye h et al., 2005 )

jsIs1 Psnb-1::snb-1::GFP P neuronal presynaptic terminals ( Nonet, 1999 )

jsIs37 Pmec-7::snb-1::GFP Touch neurons presynaptic terminals LGV ( Nonet, 1999 )

jsIs42 Punc-4::snb-1::GFP SAB, DA, I5, VA, AVF VC neurons

presynaptic terminals

( Nonet, 1999 )

juIs1 Punc-25::snb-1::GFP DD and VD neurons presynaptic

terminals

LGIV ( Hallam and Jin, 1998 )

kyIs105 Pstr-3::snb-1::GFP ASI neurons presynaptic terminals LGV ( Crump et al., 2001 )

kyIs235 Punc-86::snb-1::YFO

Punc-4::lin-10::dsRed

HSN neurons synapses LGV ( Shen and Bargmann, 2003 )

kyIs439 Podr-3::GFP::unc-2, Podr-

3::mCherry::rab-3

AWC neuron presynaptic terminal
and active zones

( Saheki and Bargmann, 2009 )

kyIs442 Podr-3::GFP::unc-2, Podr-

3::mCherry::rab-3

AWC neuron presynaptic terminal
and active zones

( Saheki and Bargmann, 2009 )

kyIs479 Punc-25::GFP::UNC-2,

Punc-25::mCherry::rab-3

DD and VD neuron presynaptic
terminals and active zones

( Saheki and Bargmann, 2009 )

nuIs24 Pglr-1::GLR-1::GFP AVG AVJ DVC PVC PVQ RIG RIS

RMD RME postsynaptic region

( Rongo et al., 1998 )

nuIs152 Punc-129::snb-1::GFP Motor neurons presynaptic terminals ( Sieburth et al., 2005 )

nuIs125 Pglr-1::snb-1::GFP AVG AVJ DVC PVC PVQ RIG RIS

RMD RME presynaptic terminals

( Juo and Kaplan, 2004 )

nuIs283 Punc-25::RFP-rab-3;Pmyo-

3::GFP GABAaR

DD VD neurons synapses ( Vashlishan et al., 2008 )

oxIs22 Pmyo-3::GFP GABAAR Motor neurons postsynaptic region ( Richmond et al., 1999 )

wyIs45 Pttx3::GFP::rab3 AIY neurons presynaptic terminals ( Colon-Ramos et al., 2007 )

wyIs85 Pitr-1 pB::GFP::rab-3 DA 9 neurons presynaptic terminals ( Klassen and Shen, 2007 )

wyIs92 Pmig-13::snb-1::YFP DA 9 neurons presynaptic terminals ( Klassen and Shen, 2007 )

wyIs109 Pmig-3::CFP::rab-3; Pmig-

13::snb-1::YFP;Pmig-
13::sng-1::mCherry

DA-9 neurons presynaptic terminal
synapses

( Poon et al., 2008 )