Biology Reference
In-Depth Information
CHAPTER 15
Molecular and Genetic Approaches for the
Analysis of C. elegans Neuronal
Development
Dong Yan and Yishi Jin
Howard Hughes Medical Institute, Neurobiology Section, Division of Biological Sciences, University of
California, San Diego, La Jolla, California, USA
I. Introduction
A. Overview of Nervous System Anatomy
B. Overview of Nervous System Development
II. Strategies for In Vivo Labeling of Neurons and Compartments
A. Constructing Transgenic Reporters to Visualize Neuronal Morphology
B. Labeling Neuronal Proteins and Subcellular Structures
C. Complex Strategy for Labeling and Manipulating Unique Neurons
D. Questions and Answers
III. Other Methods for Examining the Development of Neurons
A. Dye-Filling
B. Immunocytochemistry
C. Electron Microscopy (EM)
IV. Functional Dissection of Signaling Pathways Controlling Neuronal Development
A. Phenotypic Characterizations
B. Identifying Mutants in Forward Genetic Screens
C. Gene Expression Profiling of Neurons
D. Laser Surgery of Cells and Processes
E. Questions and Answers
V. Examples of Neuronal Development
A. Neuronal Fate Specification and Differentiation
B. Axon Guidance and Dendrite Morphogenesis
C. Synapse Formation and Specificity
D. Maintenance of Neuronal Architecture
VI. Outlook
References
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