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Fig. 8 (A) A transmitted light image of live ajm-1(ok160);ajm-1::gfp embryos embedded in agar. An embryo lacking ajm-1
function has developed a characteristic vacuole (arrow). (B) A maximum intensity projection of AJM-1::GFP expression in the
same embryos. Transmitted light and fluorescent images were obtained simultaneously on a Bio-Rad 1024 confocal using
488 nm excitation. Embryos that have lost the transgene can be easily distinguished from those that express it. (C) ATEM image
of the same embryos after HPF and embedding in Epon. The fluorescence signal can be superimposed on the low-magnification
transmission electron micrograph. The inset shows the small area within the box. The arrow points to epithelial cell membrane
separations associated with the loss of ajm-1 function. Bar = 5 m m. (For color version of this figure, the reader is referred to the
web version of this topic.)
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