Biology Reference
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needles. (C) At a higher magnification, embryos are sorted and grouped using an eyelash. (D) Embryos
and M9 buffer are transferred using a mouth pipette. (E) Three slides are placed on the bench and the outer
two are taped down to the bench. (F) A drop of molten 5% agar is placed onto the middle slide. A fourth
slide is then placed perpendicular to the three original slides. The top slide is compressed over the taped
slides. (G) The finished slide is sealed with VALAP. Using a toothpick to make hash marks in the VALAP
(arrows) aids in finding the grouping of embryos on the compound microscope. (H) A low- magnification
view of embryos within a completed mount. Embryos are grouped tightly together (image courtesy of T.
Loveless).
Fig. 1
Making a standard agar mount. (A, B) Gravid hermaphrodites are cut in half with 27
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