Biology Reference
In-Depth Information
CHAPTER 14
Imaging Embryonic Morphogenesis
in C. elegans
Jeff Hardin
Department of Zoology, University of Wisconsin-Madison, Madison, Wisconsin, USA
Abstract
I. Introduction
II. Methods
A. Mounting Embryos for Imaging Morphogenesis
B. 4D Nomarski Imaging of Morphogenesis
C. Fluorescence Imaging of Morphogenesis
D. Correlative Fluorescence and TEM During Morphogenesis (see Sims and Hardin,
2007 for more details)
III. Summary
Acknowledgments
References
Abstract
The Caenorhabditis elegans embryo is well suited to morphogenetic analysis via
modern microscopy, due to its short generation time, transparency, invariant lineage,
and the ability to generate transgenic embryos expressing various fluorescent pro-
teins. This chapter provides an overview of microscopy techniques for imaging
embryonic morphogenesis, including making agar mounts, capturing four-dimen-
sional (4D) data using Nomarski microscopy, imaging of actin in embryos, factors
important for optimizing 4D fluorescence microscopy, and recent techniques that
leverage fluorescence microscopy for intracellular imaging of cellular components
during morphogenesis.
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