The Genetics and Cell Biology of Fertilization - Caenorhabditis Elegans: Molecular Genetics and Development - page 359

Fig. 8 Examples of sperm and oocyte protein localization. (A-C) Localization of 1CB4 and SPE-9.
(A) Nomarski DIC micrograph of mature spermatozoa. (B) Localization of 1CB4 to MOs. (C)
Localization of SPE-9 to pseudopods. (D-F) Colocalization of 1CB4 and SPE-38 in spermatids. (D)
Nomarski DIC micrograph of spermatids. (E) Localization of 1CB4 to MOs. (F) Localization of SPE-38
toMOs. Note the identical distribution of staining in panels E and F. (G) GFP fluorescence of a GFP:EGG-
1 fusion protein in oocytes.
McNally, 2005 ). Destruction of the remaining cyclin B was found to require fertil-
ization and passage through the second meiotic division and more specifically, a
second E3 ubiquitin complex, Cul-2/Zyg-11, which allows progression to anaphase
II ( Liu et al., 2004; Sonneville and Gonczy, 2004 ). The use of mat-1-depleted
animals and cyclin B:GFP will help place the functioning of many genes or proteins
of interest in the context of meiotic progression. The coordination of the cell cycle,
fertilization, and egg activation are still poorly understood. The concurrent and
dependent processes of meiotic resumption and fertilization and their subsequent