Specialized Chromosomes and Their Uses in Caenorhabditis elegans - Caenorhabditis Elegans: Molecular Genetics and Development - page 23

CHAPTER 2
Specialized Chromosomes and Their
Uses in Caenorhabditis elegans
Martin R. Jones, Zoe Lohn and Ann M. Rose
Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia, Canada
Abstract
I. Introduction
A. Nomenclature for Chromosomal Rearrangements
II. Chromosomal Rearrangements
A. Translocations
B. Inversions
C. Deletions (Deficiencies)
D. Duplications
III. Characterization of Chromosomal Rearrangements
A. Restriction Enzyme Digestion of Single Nucleotide Polymorphisms (snip-SNP)
B. Oligonucleotide Array Comparative Genome Hybridization (aCGH)
C. Inverse PCR
D. Whole Genome Sequencing
IV. Engineered Constructs: Transgenic Arrays
A. Experimental Applications of Transgenic Arrays
B. Methods for Construction of Transgenic ArraysCloning
C. Properties for Construction of Transgenic Arrays
D. Methods for Creating Transgenic Animals
E. Use of Transgenic Arrays as Balancers
Acknowledgments
References
Glossary
Abstract
Research on Caenorhabditis elegans involves the use of a wide range of genetic and
molecular tools consisting of chromosomal material captured and modified for spe-
cific purposes. These ''specialized chromosomes'' come in many forms ranging from
relatively simple gene deletions to complex rearrangements involving endogenous