Biology Reference
In-Depth Information
CHAPTER 13
The Genetics and Cell Biology
of Fertilization
Brian D. Geldziler
*
, Matthew R. Marcello
*
,
z
, Diane C. Shakes
y
and Andrew Singson
*
*
Waksman Institute, Rutgers University, Department of Microbiology and Molecular Genetics, Piscataway,
New Jersey, USA
y
College of William and Mary, Department of Biology, Williamsburg, Virginia, USA
z
UMDNJ-Robert Wood Johnson Medical School, Piscataway, New Jersey, USA
Abstract
I. General Introduction/Background
II. Finding Sterile Mutants
III. Maintaining Sterile Mutants
IV. Fecundity Analysis
V. Ovulation/Ovulation Rates
VI. Spermatogenesis
VII. Sperm Migratory Behavior/Tracking
VIII. Sperm Competition
IX. Oogenesis and Oocyte Maturation
X. Assessing Fertilization and Egg Activation in Egg-Sterile Mutants
XI. Eggshell Production
XII. Analysis of Fertilization-Specific Gene Products
XIII. Future Prospects/Issues
Acknowledgments
References
Abstract
Although the general events surrounding fertilization in many species are well
described, the molecular underpinnings of fertilization are still poorly understood.
Caenorhabditis elegans has emerged as a powerful model system for addressing the
molecular and cell biological mechanism of fertilization. A primary advantage is the
ability to isolate and propagate mutants that effect gametes and no other cells. This
chapter provides conceptual guidelines for the identification, maintenance, and
experimental approaches for the study fertility mutants.
METHODS IN CELL BIOLOGY, VOL 106
Search WWH ::
Custom Search