Mapping Mutations in C. elegans - Caenorhabditis Elegans: Molecular Genetics and Development - page 20

Fig. 4 Selecting for recombination in a given interval in order to separate candidate new-1(*) muta-
tions. The actual mutation corresponding to new-1(*) is likely to be flanked by other mutations induced by
the mutagenesis (or occurring spontaneously during strain propagation). Sites of sequence discrepancy
between the dpy(-) unc(-) marker chromosome and the new-1(*) chromosome are indicated by open and
filled circles, respectively. Recombination events in intervals B and C permit the bona fide new-1(*)
mutation to be distinguished from background mutations.
21 recombinants are picked, the likelihood that at least one has a breakpoint between
these two candidate mutations is 1 - (0.857) 21 = 0.96.
Acknowledgments
The author is grateful to Barbara Conradt, David Fay and Andy Singson for constructive comments on
the manuscript, Jess Whitaker and Corinne Pierce for testing ARMS-PCR conditions, and Oliver Hobert
for helpful discussions and providing unpublished information.
References
Albertson, D. G. (1984). Localization of the ribosomal genes in Caenorhabditis elegans chromosomes by
in situ hybridization using biotin-labeled probes. EMBO J. 3, 1227-1234.
Anderson, P. (1995). Mutagenesis. Methods Cell Biol. 48, 31-58.