Affinity Purification of Protein Complexes in C. elegans - Caenorhabditis Elegans: Molecular Genetics and Development - page 312

M9

10 g NaCl
12 g Na 2 HPO 4
6gKH 2 PO 4
0.5 g MgSO 4 7H 2 O
Add ddH 2 O to 2 L and autoclave 35 min.
2 M NaOH
Bleach

Fischer Cat. #SS290 (parafilm when not in use to minimize exposure to air and
store at 4 ˚C)

OP-50

B. Large-Scale Liquid Culture
60% (w/v) sucrose in M9
0.1 M NaCl
OP-50-1

streptomycin resistant E. coli strain

LB (2 x)

100 g of LB
1800 mL ddH 2 O
Autoclave 35 min

LB + 50 m g/mL streptomycin
S Basal (1 L)

5.9 g NaCl
50 mL of 1 M KH 2 PO 4 (pH 6.0)
Add ddH 2 Oto1L
Split into two 500 mL bottles; to each bottle add 0.5 mL cholesterol (5 mg/
mLin EtOH; should form a light cloudy precipitate).
Autoclave and store at RT.

Trace Metals Solution

Disodium EDTA 1.86 g (5 mM)

FeSO 4 7H 2 O 0.69 g (2.5 mM)

MnCl 2 4H 2 O 0.2 g (1 mM)

ZnSO 4 7H 2 O 0.29 g (1 mM)

CuSO 4 5H 2 O 0.025 g (0.1 mM)

Dissolve in 1 L water; filter sterilize, aliquot into 50 mL conicals, and store in dark.