Transgenesis in C. elegans - Caenorhabditis Elegans: Molecular Genetics and Development - page 170

Table IV
Markers used to identify transgenics

Marker Plasmid Notes

rol-6(su1006) pRF4 Confers a dominant right-handed Roller phenotype to animals

( Kramer et al., 1990 ). Male Rollers do not mate well. Plasmid
available from most C. elegans laboratories

unc-119 rescue pDP#MM016B or

Cb-unc-119(+) in
transgene plasmid

Rescues uncoordinated unc-119 mutants to a wild-type phenotype
( Maduro and Pilgrim, 1995; Maduro and Pilgrim, 1996 ).
Mutant strain unc-119(ed4) available from the Caenorhabditis
Genetics Center (CGC). For use with microparticle
bombardment unc-119(+) is usually included in the transgene
plasmid. For MosSCI, the C. briggsae gene is inserted into the
targeting vector. Plasmid is available from the Maduro lab
(University of California, Riverside, CA)

lin-15 rescue pEKL15 Rescues temperature-sensitive multivulva (Muv) phenotype of

lin-15(n765) ( Clark et al., 1994 ). The lin-15(+) plasmid
pEKL15 is available from the Horvitz laboratory
(Massachusetts Institute of Technology, Cambridge, MA).
Strains harboring lin-15(n765) are available from the CGC

pha-1 rescue pBX or pC1 Rescues larval lethality of pha-1(ts) mutants to a wild-type

phenotype. Strain is maintained at 15 C, used at 25 C. Mutant
strain pha-1(e2123) is available from the CGC ( Kramer et al.,
1990 )

dpy-20 rescue pMH86 Rescues strong Dumpy phenotype of dpy-20(ts) mutants to awild-

type phenotype. Mutant strain dpy-20(e1282ts) available from
the CGC ( Clark et al., 1995 ). The plasmid is available from the
Han lab (University of Colorado, Boulder, CO)

spe-26 rescue pJV145 Rescues spe-26(hc138ts) (H. Smith and S. Ward, personal

communication; Praitis, 2006 )

Puromycin resistance pBCN21-R4R3 or

pBCN22-R4R3

Plasmids confer resistance to puromycin ( Semple et al., 2010 )

G418/neomycin resistance pdestDD04Neo,

pdestRG5271Neo,
pdestRG5273Neo

Resistance to G418 (neomycin) ( Giordano-Santini et al., 2010 )

myo-2::mCherry pCFJ90 Expresses mCherry in pharynx muscle. Plasmid available from

Addgene

elt-2::NLS::GFP::lacZ pJM66 Expresses GFP in intestinal nuclei ( Fig. 3D ). Plasmid available

from McGhee Lab (University of Calgary, AB)

sur-5::GFP pTG96 Expresses GFP in all nuclei. Plasmid available from the Han lab

(University of Colorado, Boulder, CO)

let-858::GFP pBK48.1 Expresses GFP in all nuclei. Plasmid available from Kelly Lab

(Emory University, Atlanta, GA)

unc-119::GFP pDP#MMUGF12 Expresses GFP throughout nervous system and in some head

muscles ( Fig. 3E ). GFP, YFP, CFP, and mCherry versions of
this reporter are available from the Maduro lab (University of
California, Riverside, CA)