Transgenesis in C. elegans - Caenorhabditis Elegans: Molecular Genetics and Development - page 165

Table I
Internet links (current as of May, 2011)

Website Web host Method/notes

http://www.wormbase.org WormBase Contains information about C. elegans

genes, including sequences

http://wiki.wormbase.org/index.php/
Cosmids/YACs

WormBase Information about obtaining C. elegans

clones

http://www.wormbook.org/chapters/
www_transformationmicroinjection/
transformationmicroinjection.html

WormBook - WormMethods C. elegans microinjection.

Excellent step-by-step instructions on
microinjection and microparticle
bombardment procedures

http://www.wormbook.org/chapters/
www_reportergenefusions/
reportergenefusions.html

WormBook - WormMethods An excellent description of techniques to

generate reporter gene fusions

http://www.wormbook.org/chapters/
www_transgenic/transgenic.html

WormBook - WormMethods Considerations for generation of transgenes

that express in the germ line

http://worfdb.dfci.harvard.edu/ ORFeome Source of C. elegans ORFs

http://www.geneservice.co.uk/products/
clones/Celegans_Prom.jsp

Promoterome The library contains 6000 predicted

promoters, available from Source
Bioscience

http://wormbase.org/db/searches/
expr_search

WormBase Expression pattern search tool. Can be used

to identify promoters active in particular
cells or tissues

http://nematode.lab.nig.ac.jp/db2/index.
php

Nematode Expression Pattern
Database (NEXTDB)

Contains in situ expression patterns for a
large number of genes

http://www.cbs.umn.edu/CGC/ Caenorhabditis Genetics Center

at University of Minnesota

Source for many of the strains used in
transgenesis experiments

http://sites.google.com/site/
jorgensenmossci/Home

Jorgensen Lab, Utah Mos Single Copy Insertion (MosSCI).

Detailed protocol on plasmid
construction and screening methods

http://www.addgene.org Addgene Source for many of the plasmids described

here, including for MosSCI

http://www.faculty.ucr.edu/ mmaduro/
int.html

Maduro Lab, UC Riverside, CA Summary of integration techniques using

gamma rays, chemical mutagenesis, or
UV treatment

http://www.med.yale.edu/mbb/koelle/
protocols/protocol_integrating_array.
html

Koelle Lab, Yale School of
Medicine, New Haven, CT

Step-by-step integration protocol using
gamma rays or X-rays

http://www.addgene.org/pgvec1?
f=c&cmd=showcol&colid=1

Addgene Links to documentation for Fire Lab

plasmids

C. Rescue of a Chromosomal Mutation
The C. elegans system is powerful primarily because of its genetics. For recessive
mutations, transgenes carrying the wild-type version of a gene should be able to
complement the mutation. For dominant mutations, a transgene carrying the dom-
inant allele should be able to confer a similar phenotype onto an otherwise wild-type
strain. Both of these strategies are used for marking transgenes in transformation