Mapping Mutations in C. elegans - Caenorhabditis Elegans: Molecular Genetics and Development - page 3

CHAPTER 1
Mapping Mutations in C. elegans
Eric J. Lambie
Biology Department II, Ludwig-Maximilians-University, Munich, Germany
Abstract
I. Introduction
II. Mutant Origination
III. Mutations That Can Be Propagated as Homozygotes
A. Preliminary Mutant Characterization
B. Mapping Crosses
IV. Mutations That Cannot Be Propagated as Homozygotes
A. Mapping with an Unbalanced Mutation
B. Mapping with a Balanced Mutation
V. Modifier Mutations
A. Types of Modifiers
B. Initial Characterization
C. An Example: Mapping Suppressors of gon-2(q388)
D. General Strategies for Modifier Mutations
VI. SNP Mapping
A. Bulked Lysate Analysis
B. After Bulked Lysates
VII. WGS and Beyond
Acknowledgments
References
Abstract
At present, the principal goal of mapping is to establish correspondence between a
mutation identified via a change in phenotype and an alteration in the DNA sequence
of the genome. Recent advances in molecular biology and bioinformatics have greatly
facilitated this procedure, but certain standard methods, such as the three-factor cross,
continue to be extremely useful for high-resolution mapping and separation of tightly
linked mutations. This chapter provides both general guidelines and specific proce-
dures for the characterization and mapping of newly isolated mutations in C. elegans.
Procedures are included for dealing with mutations that cannot be propagated as