Biology Reference
In-Depth Information
11. Benchtop Sorvall Legend RT Centrifuge with adapters for plate centrifugation
12. 15-25 C Percival Worm incubators
13. 37 C bacteria incubator
14. 37 C shaking incubator
15. Small rocking platform
16. Matrix WellMate (ThermoFisher) + Tubing cassettes
17. Aquarius (Tecan)
18. Corning aluminum sealing tapes for 96-well plates (#6570)
19. Abgene transparent adhesive plate seals (#AB-0580)
20. Clorox Germicidal Bleach (6.15% sodium hypochlorite), from Fisher.
21. Surveyor software (Media Cybernetics Inc.)
22. Leica stereoscopic microscopes with Luld Bioprecision stage and adaptors
Acknowledgments
The authors thank Eliana Munarriz for suggestions to the protocol, Kris Gunsalus, Katherine Erickson,
and Annalise Paaby for critical reading and editing of the manuscript, Carrie Nygard and Gina Destefano
for proofreading, and Amelia White and Huey-Ling Kao for suggestions to the figures. This work has
been supported by NIH grant #5R01HD046236-08.
References
Ahringer (ed.), J. (2006). Reverse Genetics. the C. elegans Research Community. Wormbook. Wormbook.
1551-8507.
Ben-Yakar, A., and Bourgeois, F. (2009). Ultrafast laser nanosurgery in microfluidics for genome-wide
screenings. Curr. Opin. Biotechnol. 20 , 100-105.
Ben-Yakar, A., Chronis, N., and Lu, H. (2009). Microfluidics for the analysis of behavior, nerve regen-
eration, and neural cell biology in C. elegans. Curr. Opin. Neurobiol. 19 , 561-567.
Boone, C., Bussey, H., and Andrews, B. J. (2007). Exploring genetic interactions and networks with yeast.
Nat. Rev. Genet. 8 , 437-449.
Burns, A. R., Kwok, T. C., Howard, A., Houston, E., Johanson, K., Chan, A., Cutler, S. R., McCourt, P., and
Roy, P. J. (2006). High-throughput screening of small molecules for bioactivity and target identification
in Caenorhabditis elegans. Nat. Protoc. 1 , 1906-1914.
Byrne, A. B., Weirauch, M. T., Wong, V., Koeva, M., Dixon, S. J., Stuart, J. M., and Roy, P. J. (2007). A
global analysis of genetic interactions in Caenorhabditis elegans. J. Biol. 6 ,8.
Costanzo, M., Baryshnikova, A., Bellay, J., Kim, Y., Spear, E. D., Sevier, C. S., Ding, H., Koh, J. L.,
Toufighi, K., Mostafavi, S., Prinz, J., St Onge, R. P., VanderSluis, B., Makhnevych, T., Vizeacoumar, F.
J., Alizadeh, S., Bahr, S., Brost, R. L., Chen, Y., Cokol, M., Deshpande, R., Li, Z., Lin, Z. Y., Liang, W.,
Marback, M., Paw, J., San Luis, B. J., Shuteriqi, E., Tong, A. H., van Dyk, N., Wallace, I. M., Whitney, J.
A., Weirauch, M. T., Zhong, G., Zhu, H., Houry, W. A., Brudno, M., Ragibizadeh, S., Papp, B., Pal, C.,
Roth, F. P., Giaever, G., Nislow, C., Troyanskaya, O. G., Bussey, H., Bader, G. D., Gingras, A. C., Morris,
Q. D., Kim, P. M., Kaiser, C. A., Myers, C. L., Andrews, B. J., and Boone, C. (2010). The genetic
landscape of a cell. Science 327 , 425-431.
Davierwala, A. P., Haynes, J., Li, Z., Brost, R. L., Robinson, M. D., Yu, L., Mnaimneh, S., Ding, H., Zhu,
H., Chen, Y., Cheng, X., Brown, G. W., Boone, C., Andrews, B. J., and Hughes, T. R. (2005). The
synthetic genetic interaction spectrum of essential genes. Nat. Genet. 37 , 1147-1152.
Doitsidou, M., Flames, N., Lee, A. C., Boyanov, A., and Hobert, O. (2008). Automated screening for
mutants affecting dopaminergic-neuron specification in C. elegans. Nat. Methods 5 , 869-872.
Fernandez, A. G., Gunsalus, K. C., Huang, J., Chuang, L. S., Ying, N., Liang, H. L., Tang, C., Schetter, A.
J., Zegar, C., Rual, J. F., Hill, D. E., Reinke, V., Vidal, M., and Piano, F. (2005). New genes with roles in
Search WWH ::




Custom Search