RNAi Methods and Screening: RNAi Based High-Throughput Genetic Interaction Screening - Caenorhabditis Elegans: Molecular Genetics and Development - page 105

Fig. 5 Example of a positive enhancement interaction. (A) Control for the mutation: zyg-8(b235)
L4440 (RNAi) at 22.5 C is not lethal. (B) Control for RNAi of egl-27: egl-27 (RNAi) on N2 at 22.5 Cis
not lethal. (C-D) Lethality observed for the interaction zyg-8(b235) egl-27 (RNAi) at 22.5 C repeats A
and B, respectively. Insets are enlarged sections of the pictures to show details.
no detectable increase in survival as ''not suppressed'' (NS) and place putative
suppressors into one of the four categories based on the number of larvae produced:
''strong'' (SUP, numerous larvae), ''medium'' (sup, more larvae than the control),
''weak'' (sup?, few larvae but more than the control), or ''post-embryonic'' (PE). As
for scoring enhancers, we perform secondary screening of all putative strong,
medium, and post-embryonic suppressors identified in at least one replicate and
of putative weak suppressors identified in both replicates. Fig. 6 shows an example
of a suppression interaction.
2. Automated Computerized Image Analysis
Visual inspection can be very laborious when the scale is too large. Some algo-
rithms are being developed for similar but not identical images ( Fontaine et al.,
2006; Geng et al., 2004; Huang et al., 2006; O ' Rourke et al., 2009 ). An algorithm for
the automated analysis of images is being developed in our lab to streamline this
process and provide quantitative results ( White et al., 2010 ).