Biotechnological Applications of Hemicellulosic Derived Sugars: State-of-the-Art - Sustainable Biotechnology: Sources of Renewable Energy - page 73

Ta b l e 2 Hemicellulase titers from different microorganisms and their mechanistic applications ( Source : Howard et al. [29].)

Specific activity
( mol min -1 mg -1 ) Mechanistic applications Microorganism Enzyme Substrate μ

Bacteria
Fibrobacter succinogenes Acetyl xylan esterase Acetylxylan/alpha-naphthyl

acetate

2,933 Hydrolyze the acetyl

substitutions on xylose
moieties

Thermoanaerobacter
ethanolicus

Beta-1,4-xylosidase o-nitrophenyl-beta-D-

xylopyranoside

1,073 Hydrolyse xylobiose; release

xylose

Bacillus polymyxa Beta-Glucosidase 4-nitrophenyl-beta-D-

glucopyranoside

2,417 Act upon Beta-Glucosidase to

release glucose

Bacillus subtilis Endo-alpha-1,5-arabinanase 1,5-alpha-L-arabinan 429 hydrolase activity, hydrolyzing

O-glycosyl compounds

Escherichia coli alpha-Galactosidase Raffinose 27,350 Hydrolyzes the terminal

alpha-galactosyl moieties
from xylans

Clostridium stercorarium Feruloyl esterase Ethyl ferulate 88 Hydrolyze the ester bond

between the arabinose
substitutions and ferulic acid

Bacillus subtilis Endo-galactanase Arabinogalactan 1,790 Release of L-arabinose

substituted
D-galactooligosaccharides
from arabinogalactan

Bacillus subtilis Endo-beta-1,4-mannanase Galactoglucomannan/

glucomannans/mannan

514 Acts upon interior side of

beta-1,4-mannan to yield
mannose

Fungi
Phanerochaete chrysosporium Alpha-Glucuronidase 4-O-methyl-glucuronosyl-

xylotriose

4.5 Hydrolyses Alpha-1,2

Glycosidic bond the
4-O-methyl-D-glucuronic
acid sidechain of xylans