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Fig. 3 DGGE profile of the sample from day 0 to 24 in the large-scale reactor
is dominant in an environment in which it can proliferate easily. Secondly, since
decomposing waste attached to the surface of plastic bottle flakes is accompanied
by decomposing organic waste, there is an anaerobic or microaerophilic condition
between attached waste and plastic bottle flakes, i.e., aerobic, anaerobic, aerotol-
erant and microaerophilic microorganisms might coexistent in the reactor. Thirdly,
the size of plastic bottle flakes is bigger than the woodchips. This may be inhomo-
geneous distribution, in which mixed aerobic and anaerobic conditions would occur
partially in the reactor. Or because of density depression, heat insulation capacity of
plastic bottle flakes was less than the woodchips [31].
In the small-scale reactor (reactor C) and in the large-scale reactor, operated with
plastic bottle flakes as bulking agent, the order Lactobacillales also dominated with
Bacillales , regardless of the reactor size (Figs. 1, 2, and 4). In the large-scale reactor,
DGGE analysis showed that the bacterial community changed throughout the oper-
ation (Fig. 3). All of the bands before day 11 were of the order Lactobacillales
Fig. 4 Structure of bacterial community at day 0, 7, 16 and 21 in the large-scale reactor. Clones
are classified by order level
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